Read e-book online Advances in Low-Temperature Biology, Volume 3 PDF

By Peter L. Steponkus

ISBN-10: 0080552285

ISBN-13: 9780080552286

ISBN-10: 0762301600

ISBN-13: 9780762301607

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1992). , 1966; 1981). It would seem reasonable, therefore, on the basis of the established principles for isolated tissue preservation, to attempt to develop a technique for universal preservation of all the tissues of the body during a three-hour period of HCA. , heart and CNS, and three hours would provide a generous window of opportunity for surgical intervention in the most complex of cases. j. M. E. BAILES Having demonstrated the feasibility of the UHBS approach in a canine model, we have subsequently attempted to resolve the variability of outcome reported in many of the earlier attempts to use an asanguineous strategy by devoting particular attention to optimizing the composition of the acellular perfusate used to replace the blood.

At days 3 and 7 post-op, NDS were: Gpl = 0 ± 0 vs. 0. , 1995). Only two of the dogs were successfully revived using aggressive cardiac resuscitation measures involving high doses of cardiotonic agents and repeated electroversion shocks. The physiological and neurological recovery of these survivors was manifestly slower than the experimental dogs in Group I. Figure 10 shows that neurological deficits were recorded for Group II in the first post-operative week. Problems such as hind-limb weakness or decreased vision were clearly apparent in the Group II survivors but were not detected in Group I dogs, which all demonstrated full recovery of motor functions within the first post-operative day.

In principle, this technique could provide a number of potential benefits over and above the obvious avoidance of the bloodrelated complications. In addition to total vascular and capillary washout and the removal of harmful catabolic products, blood substitution provides the opportunity to control the extracellular environment directly and the intracellular milieu indirectly as we describe in more detail below. Development of the Technique of Ultraprofound Hypothermia and Blood Substitution (UHBS) Phase /: Feasibility Study in a Canine Model using First-Generation^ Hypothermic Blood Substitutes Choice of experimental model.

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Advances in Low-Temperature Biology, Volume 3 by Peter L. Steponkus


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